Monthly Archives: January 2017

Beyond the Hofmeister Series

Why are some salts more active in protein precipitation than others? Why do we find always the same salt series in a plethora of effects. In our recent Feature Article we provide the answers and summarize the knowledge on salt-specific effects, based on the combination of thermodynamic and spectroscopic methods with computational modelling and theory in the last 10 year. link


Colorful effects of guanidinium salts on biomolecules make it to JACS

Already pure guanidinum chloride (GndCl) solution is remarkable. It contains a significant fraction of guanidinium cations in direct contact, a typically rare feature, which is counterintuitive (on electrostatic grounds) link.gndx_toc_jacs

Proteins are unfolded and solutes destabilized in concentrated solutions of GndCl in general. Indeed GndCl is, after urea, the second most common denaturing agent. In the recent article we present different faces of guanidinium action, stabilizing as well as destabilizing which are intimately connected with the anion in action. This work is a joint experimental (spectroscopy & thermodynamics) computational (simulations & theory) study. link